Figure 1
From: Painful nerve injury increases plasma membrane Ca2+-ATPase activity in axotomized sensory neurons
PMCA regulates [Ca2+ ] c in sensory neurons at rest and after activation. Sample traces demonstrate that blocking PMCA by switching from bath solution with pH 7.4 to pH 8.8 increases resting [Ca2+]c levels (A), whereas time alone has no effect (B). Transients induced by application of high K+ solution (50 mM for 0.3 s, arrows) are prolonged by pH 8.8, as measured by recovery rate constant (τ) and by the time to achieve 95% recovery to baseline (T95), as shown in the sample traces (A) and summary data (C). Time alone minimally prolongs τ but not T95 (B, D). Insets in panels A and B show superimposed recovery traces from the first and second depolarizations after normalization to identical amplitudes. Bar graphs show mean ± SD; numbers in bars indicate n; * P < 0.05, *** P < 0.001, by paired T-test.