Figure 4

Effects of neuronal injury on resting [Ca²⁺ ] _c and activity-induced transients. Neurons harvested from the 4^th lumbar (L4) and L5 dorsal root ganglia after spinal nerve ligation were compared to control neurons (C). Injury in the L5 group decreased resting [Ca²⁺]_c (A) but not transient amplitude (B). Recovery of transients induced by application of high K⁺ solution (50 mM for 0.3 s) was accelerated in the L5 population after injury, measured by both the recovery rate constant (τ) and by the time to achieve 95% recovery to baseline (T₉₅). Mean ± SD; numbers in bars indicate n; * P < 0.05, ** P < 0.01 by ANOVA with Bonferroni’s post hoc test.