Figure 1

Spatial distribution of β-catenin in normal mouse SCDH. A1-A2: Double-staining of β-catenin and SP, a marker of lamina I and outer layer of lamina II. A1. Low power images. β-catenin is detected in the gray matter of the spinal cord. In the SCDH, β-catenin displays a predominant band. A2. Higher power images of the β-catenin and SP staining in the superficial layers of the SCDH. Relatively moderate levels of β-catenin staining are detected in SP-marked layer I (I). A band with intensive β-catenin staining is observed mainly in lamina II, which partially overlaps the SP-stained outer layer of lamina II (IIo). B1-B2. Double-staining of β-catenin and IB4, a marker of the outer layer of lamina II. B1. Low power images. B2. Higher power images of the β-catenin and IB4 staining in the superficial layers of the SCDH. The outer half of the β-catenin band overlaps with IB4 (IIo), while the inner half (IIi) does not. C1-C2: Double-staining of β-catenin and PKCγ, a marker of the inner layer of lamina II (IIi). C1. Low power images. C2. Higher power images of the β-catenin and PKCγ staining in the superficial layers of the SCDH. The inner half of the β-catenin band overlaps with PKCγ. Scale bar for A1, B1 and C1: 300 μm; Scale bar for A2, B2 and C2: 50 μm.