Figure 2

Cellular localization of β-catenin in the SCDH. A-C: Double-staining of β-catenin (A) and neuronal marker NeuN (B). Small clusters of β-catenin immunoreaction product (red) are in the cytoplasm around NeuN-labeled neuronal nuclei (arrows). Low levels of β-catenin staining are also observed around non-neuronal cell bodies (arrowheads) deep in the SCDH. Little staining is seen around NeuN in deep SCDH regions (asterisks). D-F: Double-staining of β-catenin (D) and synapsin I (E), a pre-synaptic marker. The β-catenin staining substantially overlaps with synapsin I detected in lamina II (F, arrows). G-I: Double-staining of β-catenin (G) and PSD95 (H). β-catenin staining overlapped with PSD95 staining (I, arrows). J-L: Double-staining of β-catenin (J) and CD11b (K). β-catenin was barely detectable in CD11b-postive microglial cells (L, arrows). M-O: Double-staining of β-catenin (M) and GFAP (N). β-catenin staining did not overlapped with GFAP staining (O, arrows). Insets are images at a higher magnification of the areas indicated by arrowheads, to show more clearly the spatial relation of β-catenin and various molecular markers. DAPI (blue) staining was performed to visualize all cells. Scale bar: 50 μm.