Figure 4
From: Regulation of Wnt signaling by nociceptive input in animal models
Cellular localization of Wnt3a in the superficial laminae of the SCDH. A1-A2. Double-staining of Wnt3a and NeuN. A1. Low power images. Wnt3a (red) is in NeuN-labeled neurons (green) (arrows). Some NeuN-positive cells have little Wnt3a signal (arrowheads). A2. Higher power images. B1-B2. Double-staining of Wnt3a and MAP2. B1. Low power images. Wnt3a staining (red) largely overlaps with MAP2 staining (green; arrows). B2. Higher power images. C1: Double-staining of Wnt3a and CD11b. Wnt3a (red) is not in CD11b (green)-labeled microglia (arrows). D1: Double-staining of Wnt3a and GFAP. Wnt3a (red) is not in GFAP (green)-labeled astrocytes (arrows). Insets in C1 and D1 are the higher-power images of the areas indicated by arrowheads. Scale bars for A1: 100 μm, A2: 20 μm, B1, C1 and D1: 50 μm, B2: 25 μm.