Figure 6

Up-regulation of Wnt signaling proteins in the capsaicin pain model. A. Time course of mechanical allodynia induced by intradermal (i.d.) capsaicin injection in mouse hind paws. Mice with saline injection were used as controls (vehicle). Following capsaicin administration, mechanical hypersensitivity peaked at 3 h post-injection and gradually returned to baseline (*, p < 0.05; n = 6). B-D. Protein levels of Wnt3a (B), active β-catenin (ABC, C), and total β-catenin (TBC, D) at different time points after capsaicin injection. Proteins levels gradually increased and peaked around 3 h after injection. E-F. Temporal expression profiles of Wnt5a (E) and Ror2 C (F) following capsaicin injection. The levels of both proteins transiently increased after the injection. β-actin was included as a loading control. In the summary graphs (right panels), protein levels from at least three independent experiments are presented as relative units to the vehicle controls (mean ± SEM; *, p < 0.05; one-way ANOVA).