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Figure 1 | Molecular Pain

Figure 1

From: The majority of dorsal spinal cord gastrin releasing peptide is synthesized locally whereas neuromedin B is highly expressed in pain- and itch-sensing somatosensory neurons

Figure 1

Expression of Grp , Grpr , Nmb , and Nmbr mRNA in dSC and DRG neurons. (A-H) in situ hybridization with P21 wild type mouse dSC and DRG for Grp (A-B), Grpr (C-D), Nmb (E-F), and Nmbr (G-H). dSC and DRGs are outlined with black dots. Scale bars = 50 μm. (I) RT-PCR performed on RNA acutely isolated from wild type adult mouse dSC and DRG. cDNA was amplified with primers specific for Nmb, Grp, Nmbr, and Grpr. Lower bands are cDNA amplified with primers specific for Gapdh, which served as a loading control. (J) Graphical representation of real time RT-PCR for Grp performed on dSC and DRG RNA isolated from adult mice (n = 3 animals, 4 replicates per animal). Quantification represents fold difference between dSC Grp relative to that of DRG. Relative abundance of cDNA was calculated in comparison to Gapdh housekeeping gene by the 2^(−ΔΔCт) method (see details in method).

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Molecular Pain

ISSN: 1744-8069

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