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Figure 2 | Molecular Pain

Figure 2

From: The majority of dorsal spinal cord gastrin releasing peptide is synthesized locally whereas neuromedin B is highly expressed in pain- and itch-sensing somatosensory neurons

Figure 2

In vitro characterization of anti-bombesin antiserum specificity. (A) Alignment of Bombina bombina bombesin, Mus musculus Grp, and Mus musculus Nmb peptide sequences. Black bar underneath the bombesin peptide sequence indicates the immunizing peptide used for developing the rabbit anti-bombesin antiserum. Grp and Nmb residues that share identity with the immunizing peptide are highlighted in red. (B-M) HEK293 cells were transfected with EGFP-bombesin (B-D), EGFP-Grp (E-G), EGFP-Nmb (H-J), or EGFP alone (K-M) and immunostained with anti-bombesin antiserum (red) and anti-GFP antibody (green). Scale bar = 25 μm.

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