Figure 3

Calcium responses elicited by glutamate. ( A.- B.) Average peak Ca²⁺ responses are repeatable and concentration dependent. Slices prepared from 4–6 wk CD-1 mice were superfused with aCSF at 1 ml/min. Scale bar: horizontal, Δ340/380 = 0.1; vertical, time = 60 s. Data represent average ± SEM. A. Representative tracings and average peak Ca²⁺ responses to 10-s pulses of 1 mM glutamate spaced at 10 min intervals. Glutamate elicited repeatable [Ca²⁺]_i transients. N = 4 slices. B. Representative tracings and average peak Ca²⁺ responses to increasing glutamate concentrations. N = 3 slices.