Effects of 5-HT on mIPSCs in the presence of Ba2+. (A) Typical traces of mIPSCs observed before (control), during, and after the application of 5-HT (50 μM) in the presence of Ba2+ (1 mM). (B) Cumulative mIPSCs of the inter-event interval and (C) amplitude distributions compiled from the trace indicated in (A). Both the frequency and amplitude during 5-HT was not different from the control. (D) The bar graph shows the relative of the mIPSCs frequency and amplitude (paired t-test, P > 0.05, n = 7).