Figure 4

Effects of 5-HT on mIPSCs and eIPSCs of GABAergic and glycinergic interneuron. (A) 5-HT (50 μM) increased the frequency of GABAergic mIPSCs in the presence of strychnine (St, 2 μM). Subsequent application of bicuculline (Bic, 10 μM) completely eliminated the remaining mIPSCs. (B) 5-HT (50 μM) also increased the frequency of glycinergic mIPSCs in the presence of bicuculline (10 μM). The facilitated mIPSCs were eliminated by the application of strychnine (2 μM). The bar graph shows the relative frequency and amplitude for GABAergic (C, n = 7) and glycinergic (D, n = 5) mIPSCs, respectively. (paired t-test, ** P < 0.01 and * P < 0.05). (E) 5-HT (50 μM) increased the amplitude of GABAergic (n = 8) eIPSCs with the control in the presence of strychnine (2 μM) together with CNQX (10 μM) and APV (50 μM). (F) 5-HT (50 μM) also increased the amplitude of glycinergic (n = 7) eIPSCs with the control in the presence bicuculline (10 μM) together with CNQX and APV. (G) The bar graph shows the relative amplitude for GABAergic and glycinergic eIPSCs (paired t-test, * P < 0.05 and * P < 0.05).