Effects of 5-HT2A receptor agonist and antagonist on sIPSCs. (A) Representative recording showing the effects of 5-HT2A receptor agonist TCB-2 (10 μM) mimicked the enhancing effects of 5-HT (50 μM) on sIPSCs, increasing the frequency, but not amplitude. Lower traces show sIPSCs before (left) and after (right) the application of TCB-2 in the expanded time scale. (B) Shows 5-HT2A receptor antagonist ketanserin (10 μM) on the effects of TCB-2 on sIPSCs recorded from the same neuron (A). Lower traces shows sIPSCs taken before control (ketanserin) and the after TCB-2 application in the presence of ketanserin. The bar graph shows the relative frequency (C) and amplitude (D) for TCB-2, ketanserin and TCB-2 + ketanserin, respectively (paired t-test, ** P < 0.01, n = 7).