Cutaneous innervation of wild-type and Y1472F-KI mice at postherpetic neuralgia phase. (A) PGP9.5 immunostaining of nerve fibers in the skin. Cross sections of the hindlimb skin from wild-type and Y1472F-KI mice before and days 7 and 50 after inoculation were immunostained with anti-PGP9.5 antibody (red), and the nuclei were counterstained with DAPI (green). Arrows and arrowheads indicate epidermis and nerve fibers, respectively. Scale bars = 50 μm. (B, C) Total length of PGP9.5-immunoreactive nerves (B) and the number of CGRP-immunoreactive nerve fibers (C) in the epidermis. Intraepidermal nerve fibers immunoreactive for PGP9.5 and CGRP were measured in the affected skin and contralateral skin from wild-type and Y1472F-KI mice on day 50 post-inoculation, as described in the Methods section. Data are presented as the mean ± SD (n = 6 and 8 for PGP9.5, and 5 and 6 for CGRP, in wild-type and Y1472F-KI mice, respectively). *P < 0.05 (Bonferroni t-test).