Immunoreactivities of CGRP and SP, and IB4 labeling in the dorsal horn of spinal cord at postherpetic neuralgia phase. Cross sections of the spinal cord of wild-type and Y1472F-KI mice at the postherpetic neuralgia phase (day 50) were immunostained with anti-CGRP and anti-SP antibodies and labeled with IB4. A representative image of the dorsal horn region of each mouse is shown, and insets depict higher magnification of the image delineated by the rectangles. The percentage of immunoreactive intensity in the dorsal horn (dotted line) was determined, and the data are presented as the ratio of the affected side to the contralateral one (n = 5). *P < 0.05 (two-tailed Student's t-test). Scale bars = 200 μm for low-power photos and 50 μm for high-power ones.