Figure 8From: Involvement of Tyr1472 phosphorylation of NMDA receptor NR2B subunit in postherpetic neuralgia in model mice Neurite outgrowth and neurotoxicity of DRG neurons. (A) Neurite outgrowth of DRG neurons by NGF and GDNF. L4–L6 DRGs were harvested from naive or axotomized (AX) wild-type and Y1472F-KI mice at 4–5 weeks of age. DRG neurons were grown in the presence or absence of NGF (10 ng/ml) or GDNF (10, 50 or 100 ng/ml) for 22–26 h. Neurite outgrowth, shown in the photo, is presented as a percentage of total DRG cells. Data are presented as the mean ± SD (n = 3, 4, and 2 for NGF, GDNF, and axotomy, respectively). *P < 0.05 (two-tailed Student's t-test). (B) Neurotoxicity of glutamate toward DRG neurons. DRG neurons prepared from naive wild-type and Y1472F-KI mice were incubated without or with the indicated concentrations of glutamate for 24 h. The release of LDH was measured as described in the Methods section. Experiments (n = 5–6 at each concentration) were carried out by using 3 independent preparations. Data are presented as the mean ± SD of 3 experiments. *P < 0.05 vs. Y1472F-KI mice (two-tailed Student's t-test).Back to article page