Figure 4

IL-6 enhanced direct association between ERK1 and Nav1.7. Western blot and quantification for tERK following 15 mins treatment of TG cultures with vehicle, IL-6 (50 ng/ml) or IL-6 (50 ng/ml) + U0126 (10 μM) (A) Interaction between ERK and Nav1.7 was examined by immunoprecipitation (IP) with antibody against Nav1.7 followed by immunoblotting (IB) for tERK. Omission of primary antibody was used as negative control and whole cell lysates were loaded as positive control. (B) ERK1 was quantified and values were expressed as percentage of vehicle. Significant (*p < 0.05) differences among means for each group were determined by analysis of variance followed by Newman-Keuls post hoc test.