Epidermal CGRP-IR 2-D density profile of the hind paw glabrous skin. A: Two large skin biopsies (left hand panel, grey shaded areas) are taken from the distal and proximal foot sole and cut transversely. Serial sections were plotted, binned and the density of labeled profiles was visualized in a 2-D density profile that was made using Matlab routines (colorbar reflect number of labeled fibers.) The resulting plots indicate that the footpads are less densely innervated compared to non-footpad areas. B: Quantification shows a significant lower number of epidermal CGRP-IR fibers in the footpads compared to non-footpads. (N = 5), student t-test, *P < 0.05.