Figure 3

Analysis of action potential after incubation with TGFβ1. Top: Left, a representative action potential (AP) tracing (solid line) and threshold (dotted line) taken from a DRG neuron treated with TGFβ1. The single AP was evoked by a current injection at rheobase of 0.2 nA, and the parameters were measured for AP amplitude, threshold, Action potential durations at base (APBD) widths at half amplitude (AP ½ HW). The associated trace is a derivative of AP showing shoulder duration appeared on the falling phase of the AP. Right, AP amplitudes were not different in the two groups: 72.37 ± 4.5 mV in control (n = 19) and 82.94 mV in TGFβ1 group (n = 17; P = 0.11). Bottom: Left, APBD: controls = 12.7 ± 0.94 ms (n = 19) and TGFβ1 treated neurons = 18.97 ± 1.44 ms (n = 17). Middle, AP ½ HW: controls = 4.73 ± 0.37 ms (n = 19) and TGFβ1 treated neurons = 5.89 ± 0.41 ms (n = 17). Right, Shoulder durations: controls = 2.26 ± 0.26 ms (n = 19) and TGFβ1 treated neurons = 4.36 ± 0.76 ms (n = 17).*P <0.05,**P ≤0.01.