Figure 5

a. Behavioral response (“pain behaviors”) to electrical stimulation of the pancreas 16–24 hours after intrapancreatic infusion of TGFβ1 or vehicle. The graph shows the pooled results of two replicate experiments, each of which showed a statistically significant result when analyzed independently. Intrapancreatic TGFβ1 results in a significant upward shift of the stimulus response curve (P < 0.0001 for stimulus and P < 0.01 for TGFβ1 effect by two way ANOVA; n = 10 in the TGFβ1 group and n = 9 in the vehicle group). b, c. Attenuation of pancreatic hyperalgesia (as measured by the behavioral response to electrical stimulation) in a rat model of chronic pancreatitis by intraperitoneal injection of a neutralizing antibody to TGFβ1 (left panel) or a non-neutralizing antibody also directed against TGFβ1 (right panel). Baseline responses were obtained and then repeated one week after administration of the antibody. Treatment with the neutralizing antibody resulted in a marked attenuation of the behavioral response to electrical stimulation of the pancreas as compared to baseline (P < 0.0001 for both stimulus and treatment; n = 9) whereas administration of the non-neutralizing antibody resulted in no change in the response (n = 9). d, e. Attenuation of referred somatic hyperalgesia, as measured by Von Frey filament (VFF) testing. Treatment with the neutralizing antibody resulted in a decrease in the response frequencies to abdominal wall probing as compared to baseline (P < 0.0001 for both stimulus and treatment) whereas administration of the non-neutralizing antibody resulted in no change in the response.