Figure 5From: Expression of the transient receptor potential channels TRPV1, TRPA1 and TRPM8 in mouse trigeminal primary afferent neurons innervating the dura The distribution of neurons expressing TRPV1 channels in the TG and dural afferent neuron populations. (A) Representative images of a TG section containing FG+ dural afferent neurons and neurons exhibiting TRPV1-ir. Arrowheads indicate neurons that are both FG+ and TRPV1+. (B) The percentage of TRPV1+ TG neurons in the V1/V2 and V3 divisions (n = 3 mice; on average, 730 V1/V2 neurons and 500 V3 neurons were counted from each mouse; *** p < 0.001, two-tailed t-test). (C) The percentages of V1/V2 TG neurons (the same data set as in B) and FG+ dural afferent neurons that are TRPV1+ (n = 3 mice, on average, 213 FG+ neurons were counted from each mouse; * p < 0.05, two-tailed t-test). (D) The size distribution of TRPV1+/FG+ dural afferent neurons (n = 151 neurons pooled from three mice) are similar to the TRPV1+ neurons in the V1/V2 divisions of the TG (n = 679 neurons pooled from three mice, p = 0.1, Mann–Whitney U test). (E) The sizes of TRPV1+ neurons (the same neurons as in D) are significantly smaller than those of the neurons in the V1/V2 divisions of the TG (n = 2189 neurons pooled from three mice, p < 0.001, Mann–Whitney U test). (F) The sizes of TRPV1+/FG+ dural afferents (the same neurons as in D) are significantly smaller than those of the total FG+ dural afferent neurons (n = 638 neurons pooled from three mice, p < 0.001, Mann–Whitney U test).Back to article page