Table 1 Primer pairs for real-time qPCR

Scn8a (Nav1.6)

[NCBI:NM_001077499]

153

93%

for: 5’-CGTACTATTTGACGCAGAAAACTT-3’

Exon 2- 3

rev: 5’-TCATGCTGAAGACTGAATGTATCA-3’

Exon 3- 4

Scn9a (Nav1.7)

[NCBI:NM_018852.2]

142

95%

for: 5’-CAGCAAAGAGAGACGGAACC-3’

Exon 18- 19

rev: 5’-TCTCCTCACACAGCCATCTG-3’

Exon 19- 20

Scn4b (β4)

[NCBI:NM_001013390]

116

96%

for: 5’-CGAAACATCCAGGATTCTCAT-3’

Exon 2-3

rev: 5’-TCGTCTTCTCCTTGGTGGA-3’

Exon 3

Rpl13a

[NCBI:NM_009438.5]

59

99%

for: 5’-GCCTACCAGAAAGTTTGCTTACC-3’

Exon 6-7

rev: 5’-GGTACTTCCACCCGACCTC-3’

Exon 7-8

HPRT

[NCBI:NM_013556]

132

94%

for: 5’-TGGAAAGAATGTCTTGATTGTTG-3’

Exon 4-5

rev: 5’-CGAGAGGTCCTTTTCACCAG-3’

Exon 7