Figure 1

The incidence of cold hyperalgesia (A) and expression of TRPM8 (B-E) following oxaliplatin or sodium oxalate treatment. Oxaliplatin (4 mg/kg) or sodium oxalate (1.3 mg/kg) was administered i.p. on days 1 and 2. A: The acetone test was performed on days 0, 3, 5, 8 and 15. B: On day 5 the rat L4-6 DRG treated with oxaliplatin was harvested and the mRNA expression of TRPM8 and G3PDH were determined by PCR. C: On day 5 the rat L4-6 DRG treated with sodium oxalate was harvested and the protein of TRPM8 and β-actin were determined by Western boltting. D, E: 500 μM of oxaliplatin (D) or sodium oxalate (E) was administered to cultured DRG cells for 3, 6 or 12 h. mRNA expression of TRPM8 and G3PDH was determined by PCR. Values are expressed as the mean ± SEM of 4-6 animals (A, B) or 4-6 wells (C, D). *p < 0.05, **p < 0.01 compared with vehicle or control group.