Figure 3

NFAT nuclear translocation in primary cultured DRG cells. Oxaliplatin (A, B: 500 μM for 1-6 h) or sodium oxalate (C, D: 500 μM for 1-6 h) was administered to cultured DRG cells. E, F: Mexiletine (Mex, 1 mM), nifedipine (Nif, 30 μM), diltiazem (Dil, 30 μM) or vivit (2 μM) was co-administered with sodium oxalate (500 μM) to cells for 6 h. NFATc4 immunostaining (green) and nuclear staining with DAPI (blue). NFATc4 and DAPI-positive nuclei were visualized by fluorescence microscopy (A, C, E). The nuclear translocation of NFATc4 was calculated by comparing the ratio of nuclear NFATc4 immunofluorescence/total NFATc4 immunofluorescence (B, D, F). Values are expressed as the mean ± SEM of 24-33 cells. ††p < 0.01 compared with control group, **p < 0.01 compared with oxalate group.