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Figure 4 | Molecular Pain

Figure 4

From: The cytochrome P450 inhibitor, ketoconazole, inhibits oxidized linoleic acid metabolite-mediated peripheral inflammatory pain

Figure 4

Effect of Linoleic acid on Nocifensive Behavior in Rats and Mice. A. Rats were injected either with saline or 1:1 CFA and 24 hours later, vehicle, 10, 100 or 1000ug linoleic acid was injected into normal uninflamed and/or inflamed hindpaws and nocifensive behavior was recorded upto 15 minutes. Data are plotted as total nocifensive behavior time (in seconds) within 15 minutes. n = 6 was used for each group. Data Analyzed using one-way ANOVA with Neuman-Keuls post hoc test. Error Bar: S.E.M. B. Rats were injected with 1:1 CFA and 24 hours later, 50 mg/kg capsazepine or vehicle was injected systemically underneath the neck. 1 hour later, 1000ug of LA was injected in the inflamed paws and nocifensive behavior was recorded upto 15 minutes. Data plotted is the total nocifensive time (in secs) observed between 8 and 15 minutes. n = 8 was used for each group. Data analyzed using 2-tailed Student’s t-test. Error Bar: S.E.M. C. Wild-type mice or TRPV1 KO mice were injected with 1:1 CFA and 24 hours later 1000ug LA was injected in the inflamed paws of wild-type and TRPV1 KO mice. Nocifensive behavior was recorded and plotted between 8 and 15 minutes after injection. A vehicle group was included in the TRPV1 KO mice as well. Data plotted is the total nocifensive time (in sec) observed between 8 and 15 minutes. n = 8 was used for each group. Data Analyzed using one-way ANOVA with Neuman-Keuls post hoc test. Error Bar: S.E.M.

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