Figure 2

Single and repeated injections of NAC cause analgesia in the CFA model of chronic inflammatory pain. Mice were injected with vehicle (saline) or CFA in the right hind paw, and tested for mechanical pain thresholds after 7 days. In (A) mice were treated i.p. with a single injection of NAC (100 mg/kg) or saline preceded, 15 min earlier, by saline or LY341495 (1 mg/kg). In (B), mice were treated i.p. with saline or NAC for 7 days. Either saline or LY341495 (1 mg/kg) were injected i.p. 15 min prior to the last injection of NAC or saline. In both (A) and (B), values are means ± SEM of 9 mice per group. p < 0.05 (Two-way ANOVA + Holm-Sidak’s test) vs. the respective groups of control mice treated s.c. with vehicle (saline) in the hind paw (*), or vs. all other groups of CFA mice (#).