Peripheral nerve injury (SNI) up-regulates LPCAT2 mRNA in the ipsilateral spinal cord. (A) Gel panels show PCR products from the ipsilateral L4-L5 spinal cord taken from 0 (naive), 1, 3, 7 and 14 days after nerve injury. (B) Graphs show quantification of the relative mRNA levels of LPCAT1 and LPCAT2. LPCAT1 and LPCAT2 mRNA levels were normalized against GAPDH (n = 4, mean ± SEM, #; p < 0.05 compared with naive). (C-F) Darkfield images of ISHH revealed the mRNA expression of LPCAT1 (C, D) and LPCAT2 (E, F) in naive rats (C, E) and 3 days after nerve injury (D, F). Calibration bar: 500 μm.