Figure 1

Scgn expression in DRGs. (a) Quantitative (real-time) PCR detection of Scgn mRNA in mouse olfactory bulb (OB), ventral and dorsal horns (VH/DH) of L4-5 spinal cord segments, and L4-5 DRGs. Scgn mRNA is found expressed at significantly lower levels in VH and DRGs than in the OB. Scgn mRNA is under the detection threshold in DH. Gapdh is used as internal control. (b-c) Scgn mRNA expression in human DRG (hDRG). Semitransparent purple and green shades outline the surface area of Scgn mRNA-positive and negative cells, respectively. Note that tissues are counterstained by cresyl violet after emulsion-radiography of the mRNA hybridization signal. Scgn-LI in control L5 mDRGs (d-m”). Immunofluorescence micrographs of sections incubated with antiserum to Scgn (d-m), CGRP (f’), IB4 (g’), TRPV1 (h’), GRP (i’), NF200 (j’), CB (k’), PV (l’) or CR (m’). Color micrographs show merged micrographs after double-staining (f-f”, g-g”, h-h”, i-i”, j-j”, k-k”, l-l”, m-m” show, respectively, the same section). (d) Several Scgn-IR cells are seen. Boxed area shows Scgn staining also in the nucleus. Arrowheads indicate Scgn-positive fibers. (e) Double labeling shows the same staining pattern present after incubation with two different Scgn antibodies (arrowheads). (f-m”) Arrowheads indicate coexistence of Scgn (yellow) with CGRP (f-f”), TRPV1 (h-h”), NF200 (j-j”) and CR (m-m”), most pronounced for Scgn plus CGRP (f-f”). Scgn-LI cannot be seen in IB4-positive (g-g”), GRP (i-I”), CB-(k-k”) or PV-IR (l-l”) neurons. Scale bars indicate 10 μm (b,c), 50 μm (d,e-m”).