Figure 3

Scgn-LI in hDRG and human spinal cord. (a-d) Immunofluorescence micrographs of section incubated with antiserum against Scgn (a,b,d) or/and CGRP (c,d). (b-d) show same section processed for double-immunofluorescence. Arrowheads indicate Scgn-IR neurons (a), and neurons co-expressing (solid arrowheads in d; yellow) Scgn- (b, d) and CGRP-LI (c, d). Note that all Scgn-IR neurons are CGRP-IR. (e-g) Immunofluorescence micrographs of section incubated with antiserum against Scgn (e, g) or/and IB4 (f, g). e-g show same section processed for double-immunofluorescence. Arrowheads indicate Scgn-IR fibers (e, g). Note that no Scgn-IR neuron is IB4-positive. (h-p) Immunofluorescence micrographs of a section incubated with antiserum against Scgn (h,j,k,m,n and p) or/and CGRP (i,j,l,m,o and p). h-j show the double-labeling of Scgn and CGRP in human spinal cord at low magnification. Scgn-IR fibers are concentrated in the lateral superficial layer and some of them show co-localization with CGRP as indicated by arrowheads (inset in j). k-m show Scgn-IR interneurons surrounded by some positive nerve endings in inner layer of lamina II (arrowheads indicate Scgn-IR interneurons). n-p show the co-localization of Scgn-LI and CGRP-LI in axons in the dorsal roots (arrowheads, yellow). Scale bars indicate 200 μm (a, h-j), 100 μm (b-g), 20 μm (k-m, n-p and inset in j).