Figure 5

Scgn-LI in control mouse lumbar spinal cord. (a-t) Immunofluorescence micrographs of sections incubated with antiserum against Scgn (a-g, i-t), CGRP (h, i), TRPV1 (j-j”), GRP (k, k’), PKC gamma (l, l’), CB (m, m’, r-t), PV (n, o), or/and CR (p, q). Scgn-IR fibers are seen in the superficial dorsal horn (arrowheads in a, b) and also processes in lamina III (a, b). Scgn-IR cell bodies are present in lamina II (small-sized in c), a few in III (d) or medial (multi-polar in e) and lateral (small-sized in f) parts of lamina IV. (g-i) Ventral horn neurons express Scgn-LI (g, i) and are mostly CGRP-IR (arrowhead in i). Scgn-IR (j”, k’, I’; green) neurons do not express TRPV1 (j,j”), GRP (k, k’) or PKC gamma (l, l’) in dorsal horn. (m-q) Filled Arrowheads indicate coexistence (yellow) of Scgn-LI (m’, o, q, green) and CB (m, red), PV (o; red) or CR (q; red) in the dorsal horn. (r-t) Triple-labeling of superficial dorsal horn showing (r and r’ same section) a local neuron (light blue; filled arrowhead) coexpressing Scgn (r’; green) and CB (r’; read), but not PV (r’; dark blue). s and t show neurons (filled arrowhead; light blue) containing Scgn (green) and CB (red), but not CR (dark blue). Empty arrowheads indicate a dorsal horn neuron only express Scgn-LI (j'',k',l',o,q,t) or a Scgn-IR fiber (j'; green) does not overlap with TRPV1-IR fiber (j'; red). Scale bars indicate 200μm (a), 50 μm (b=m=n=p; c=d=e=f; g=h=i; j=k=l; j’=j”; k’=l’; m’=o=q; r=s; r’=t).