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Figure 1 | Molecular Pain

Figure 1

From: JAK-STAT1/3-induced expression of signal sequence-encoding proopiomelanocortin mRNA in lymphocytes reduces inflammatory pain in rats

Figure 1

IL-4 induces POMC exon–3 mRNA expression in lymphocytes via JAK/STAT signaling. Lymphocytes were incubated for 2 h in the presence of A) IL-1β (n = 5), B) IL-4 (n = 6), and C) ConA (n = 3) at the indicated concentrations. D-F) Node cells were preincubated with indicated doses of pyridon 6 (n = 5), A77 1726 (n = 4) or cyclic pifithrin-alpha (n = 5) prior to IL-4 addition. H-I) Lymphocytes were transfected 24 h prior to the addition of IL-4 with STAT6 (n = 5), STAT5 (n = 6), and STAT1/3 (n = 6) decoy oligonucleotides at the indicated concentrations. In A-C POMC mRNA ratios are given in relation to unstimulated controls; in D-I POMC mRNA ratios are given in relation to inhibitor-free, IL-4 stimulated controls. Data represent means ± SEM. Statistical analysis was performed on raw data of A+C using the Wilcoxon signed rank test; raw data of B and D-I were analyzed using the Friedmann and Dunn’s test *P < 0.05; **P < 0.01.

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Molecular Pain

ISSN: 1744-8069

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