Flow cytometric analysis of lumbar spinal cord mononuclear cells. Total mononuclear cells collected from each sample (pooled ipsilateral or contralateral side of lumbar spinal cords from 3–4 mice) were labeled with mAbs against CD11b and CD45. All samples were examined with an Accuri C6 flow cytometer and analyzed with FlowJo. For each sample, total cell population was first identified (A), and then microglia (CD45loCD11b+) (B) and infiltrating leukocytes (CD45hi) (C) were identified within the gated total cell population. The percentage of each of these populations within the total cells was recorded. The total number of microglia and infiltrating leukocytes per spinal cord were calculated based on the recorded percentages, the total mononuclear cells collected, and the number of mice used in each sample. Representative plots shown here are from a “WT day 3 L5Tx ipsilateral” spinal cord mononuclear cell sample.