Numbers of total mononuclear cells in lumbar spinal cord following L5Tx in WT, CD4KO, and CD40KO mice. WT, CD4 KO, and CD40 KO mice were subjected to sham or L5Tx surgery. Lumbar spinal cord mononuclear cells from separate groups of mice of each genotype were collected at indicated times post-surgery. The temporal changes of the numbers of total lumbar spinal cord mononuclear cells in WT (A, n = 5–8), CD4 KO (B, n = 4) and CD40 KO (C, n = 4) mice are shown here (mean ± SEM). One-way ANOVA was performed to examine the basal level genotypic differences among naïve mice and no significant differences were found. Two-way ANOVA for data sets in each graph were performed. * indicates significant differences between the indicated group and all other groups at the same time point. # indicates significant differences between the indicated group and the corresponding Day 0 group. An additional significant result from statistical comparison is also shown within the graph. “Tx” = L5Tx, “Sh” = sham operation, “ipsi” = ipsilateral side, and “contra” = contralateral side. For naïve mice, “ipsi” = left and “contra” = right.