Mechanical hypersensitivity of WT mice treated with anti-CD40. WT mice were subjected to L5Tx. Neutralizing CD40 antibody was i.t. administered to WT mice daily either from day −1 to day 7 (A) or from day 6 to day 14 (B). Mechanical sensitivity of each mouse was tested before L5Tx and repeatedly after L5Tx using a series of von Frey filaments via the up-down method. All data are presented as mean ± SEM (n = 6). Two-way RM ANOVA was performed for data sets within each graph. * indicates the significant differences between the indicated group and the saline treated group within the same time point. In addition, within each treatment group, p < 0.05 between any time point post-L5Tx and both day 0 and day −1 time points (not indicated in the graph).