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Figure 6 | Molecular Pain

Figure 6

From: Upregulation of cystathionine beta-synthetase expression by nuclear factor-kappa B activation contributes to visceral hypersensitivity in adult rats with neonatal maternal deprivation

Figure 6

Effect of NMD on p65 translocation. Colon-innervating DRGs (T13-L2) were harvested from control (CON) and NMD rats. After cell fraction, 20 μg of nuclear and 40 μg of cytosolic fractions were used for p65 western blotting. (A) Original Western blots demonstrating expression of p65 subunit of NF-κB protein (upper) in cytosolic fractions from control (left) and NMD (right) rats. β-Actin (lower panel) was used as a loading control. Quantification of the bands by densitometry showing that the content of p65 in the cytoplasm was significantly lower in NMD group than in CON group. n=5 rats for CON, n=4 rats for NMD; *P<0.05. (B) Original Western blots demonstrating expression of p65 subunit (upper) in nuclear fractions from control (left) and NMD (right) rats. Histone 3 (H3, lower panel) was used as a loading control. Quantification of the bands by densitometry showing that the content of p65 in the nuclear was significantly higher in NMD group than in CON group. n=3 samples for each group, 4 rats in each sample; *P<0.05. Thus, NMD promotes p65 translocation from cytoplasm to nucleus in colon-innervating DRGs.

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