PDTC reduced CBS expression and attenuated visceral hypersensitivity. (A) Original Western blots demonstrating expression of CBS protein (upper) from NS (left) and PDTC (right) treated rats. β-Actin (lower panel) was used as a loading control. Quantification of the bands by densitometry showing that the intrathecal injection of PDTC at 1 μg once a day for consecutive 7 days remarkably inhibited CBS expression. n=3 rats for each group. *P<0.05. (B) Intrathecal injection of different doses of PDTC reduced the AWR scores compared with NS treatment, in a dose-dependent manner. n=8 rats for each group. *P<0.05. (C) Time course of PDTC effect. Administration of PDTC greatly enhanced the distension threshold on NMD rats for ~60 min compared with NS-treated rats. n=8 rats for each group. *P<0.05.