Figure 7From: Upregulation of cystathionine beta-synthetase expression by nuclear factor-kappa B activation contributes to visceral hypersensitivity in adult rats with neonatal maternal deprivation PDTC reduced CBS expression and attenuated visceral hypersensitivity. (A) Original Western blots demonstrating expression of CBS protein (upper) from NS (left) and PDTC (right) treated rats. β-Actin (lower panel) was used as a loading control. Quantification of the bands by densitometry showing that the intrathecal injection of PDTC at 1 μg once a day for consecutive 7 days remarkably inhibited CBS expression. n=3 rats for each group. *P<0.05. (B) Intrathecal injection of different doses of PDTC reduced the AWR scores compared with NS treatment, in a dose-dependent manner. n=8 rats for each group. *P<0.05. (C) Time course of PDTC effect. Administration of PDTC greatly enhanced the distension threshold on NMD rats for ~60 min compared with NS-treated rats. n=8 rats for each group. *P<0.05.Back to article page