Figure 4

Time course of BDNF regulation of aPKCs in spinal SNSs. SNSs were prepared from mice and treated with BDNF for indicated time points. A) BDNF transiently increased mTOR, AKT (S473) and p70S6 kinase (p70) phosphorylation but led to a longer lasting increase in AKT phosphorylation at T308 and in total levels of PKCλ, PKMζ and CaMKIIα (N = 6). B) To assess whether BDNF increased PKMζ phosphorylation, SNSs were treated with BDNF in the absence of amino acids. BDNF treatment led to a persistent increase in AKT (T308, PDK1 site) and PKMζ (T410, PDK1 site) phosphorylation (N = 6). * p < 0.05, ** p < 0.01, one way ANOVA with Bonferroni post hoc test compared to Veh.