Figure 6

BDNF activates mTOR and increases PKCλ, PKMζ and CaMKIIα protein in cortical SNSs. A) qPCR was used to assess aPKC mRNA expression in whole cortex samples (Input) and in cortical SNSs SNSs. PKMζ and PKCλ mRNA were found in cortex and cortical SNSs but not PKCζ. GluN1 mRNA was enriched in cortical SNSs (N = 3). B) SNSs were prepared from mouse cortex and stimulated with 100 ng/ml BDNF for 15 min. Treatment led to an increase in mTOR, AKT and p70S6 kinase (p70) phosphorylation as well as increased levels of total PKCλ, PKMζ and CaMKIIα (N = 6). * p < 0.05, ** p < 0.01, *** p < 0.001, student’s t-test.