Figure 7

Effect of EA on VR-1 expression in the ipsilateral spinal dorsal horn at 14 day after CFA injection. A. Expression of VR-1 mRNA (relative to GAPDH) in L4-6 ipsilateral spinal dorsal horn measured by qPCR. Standard curve of VR-1 and GAPDH mRNA in the ipsilateral spinal dorsal horn is shown in Additional file 1: Figure S4. It shows that relative quantity of VR-1 mRNA of CFA group is much more than that of Control group on day 14 after CFA injection, and EA suppresses the expression of VR-1 mRNA. *P<0.05 vs. group Control. ^{▲ ▲} P<0.01 vs. group CFA. ^● P<0.05 vs. group CFA+Sham EA. n=5-7. B, C. Western blotting analysis reveals that VR-1 protein in the L4-6 ipsilateral spinal cord dorsal horn has significant difference between Control group and CFA group, and EA suppresses the expression of VR-1 protein. C. Quantification of B. VR-1 protein in normalized against β-actin. *P<0.05, **P<0.01vs. group Control. ^▲ P<0.05 vs. group CFA. n=4-6.