Figure 2

Post- rather than pre-synaptic effect of CRF. (A) Original current traces of miniature EPSCs (mEPSCs) recorded in the presence of TTX (1 μM) in one CeLC neuron before (Predrug) and during CRF (10 nM). Scale bars, 20 pA, 200 ms. CRF (10 nM, 12 min) increased amplitude (B) but not frequency (C) of mEPSC significantly (cumulative inter-event interval distribution for individual neuron, P < 0.0001, Kolmogorov–Smirnov test; mean frequency, n = 5 neurons, P < 0.05, paired t-test). Data for each neuron were obtained from 2 predrug recording periods and 2 recording periods during CRF (5 min each period). Bar histograms show means ± SEM expressed as percent of predrug control (set to 100%). Statistical analysis was done using raw data.