Figure 7

Inhibition of PKA, but not PKC, blocks CRF effects on audible vocalizations and spinal reflexes. (A,B) Coapplication of a PKA inhibitor (KT5720, 100 μM, concentration in microdialysis probe, 15 min) decreased the facilitatory effect of CRF on audible and ultrasonic vocalizations (voc.) significantly (n = 5 rats). (C) KT5720 also inhibited the CRF effect on spinal reflex thresholds (n = 5 rats). (D,E,F) Coapplication of a PKC inhibitor (GF109203x, 100 μM, concentration in microdialysis probe, 15 min) did not block the effects of CRF on audible and ultrasonic vocalizations (n = 6 rats) and on spinal reflex thresholds (n = 5 rats). Bar histograms show means ± SEM. *,**,*** P < 0.05-0.001, compared to predrug (ACSF) before CRF; ^#,^##,^### P < 0.05-0.001, compared to CRF (15 min time point); Bonferroni posttests.