Figure 10

Immunolocalization of NCX isoforms in axons innervating dental pulp. (A, D, and G) Pan neuronal marker immunoreactivity (red) and nuclei (blue). Intense immunoreactivity was observed for Pan neuronal marker in axons innervating the dental pulp. Pan neuronal marker includes anti-NeuN, anti-MAP2, anti-βIIItubulin, and anti-neurofilament 200 (NF-H) antibodies. (B, E, and H) Immunoreactivity for NCX1 (B), NCX2 (E), and NCX3 (H). (C, F, and I) Double staining of Pan neuronal marker (red) and NCX isoforms (green). All NCX isoforms were expressed on Pan neuronal marker-positive neurons. No fluorescence was detected in the negative control (data not shown). Scale bar: 20 μm.