Figure 2

Characterization of primary TG cultures. (A) In presence of extracellular Ca²⁺ (1.5 mM; upper white box), application of 50 mM KCl solution (upper black boxes) induced an increase in [Ca²⁺]_i in TG neurons (black solid line), while no increase in [Ca²⁺]_i was observed in other types of cell in TG cultures (grey solid line). (B) Summary bar graphs showing normalized cell numbers tested. In these TG cultures, cells responding to high-KCl solution-induced depolarization with neuronal properties occupied 88.2% (black column), while other cell populations occupied 11.8% (grey column). Data points represent mean ± S.E. of number of separate experiments (numbers in parentheses represent number of tested cells). Statistically significant differences between columns are indicated by asterisk: *p<0.05.