Figure 5

Immunolocalization of NCX isoforms in somata of TG in cryosections. (A, B, and C) NCX immunoreactivity (red) and nuclei (blue). Intense immunoreactivity was observed for NCX1 (A), NCX2 (B), and NCX3 (C) on entire cell bodies of TG cells. (D) Positive immunoreactivity to NF-H as an A-neuron marker in TG neurons. (E, F, and G) Double staining of NCX isoforms (red) and NF-H (green). (H) Positive immunoreactivity to isolectin B4 (IB4; as a non-peptidergic C-neuron marker) in TG neurons. (I, J, and K) Double staining of NCX isoforms (red) and IB4 (green). (L) Positive immunoreactivity to calcitonin gene-related peptide (CGRP; as a peptidergic C-neuron marker) in TG neurons. (M, N, and O) Double staining of NCX isoforms (red) and CGRP (green). NF-H-, IB4-, and CGRP-positive neurons each expressed all 3 NCX isoforms. No fluorescence was detected in the negative control (data not shown). Scale bar: 20 μm.