Figure 7

Pharmacological identification of NCX in TG neurons. Dose-dependent inhibition of NCX by KB-R7943 (A, B), SEA0400 (C, D), or SN-6 (E, F). (A, C, and E) Ca²⁺ influx by NCX was reversibly inhibited by KB-R7943 (A) SEA0400 (C), or SN-6 (E) in concentration-dependent manner. External solution-changing protocol of [Na⁺]_o alone and for [Li⁺]_o with 1.5 mM [Ca²⁺]_o is shown in upper grey boxes in A, C, and E. Black bars in lower panels of each figure indicate times of application of these inhibitors to external solution. (B, D, and F) Dose–response relationships for inhibitory effects of KB-R7943 (B), SEA0400 (D), or SN-6 (F) on Ca²⁺ influx by NCX. Data points in each figure illustrate F/F₀ values as function of applied concentration of inhibitors and represent mean ± S.E. of number of separate experiments (numbers in parentheses represent number of tested cells). Curves (solid lines) were fitted according to Equation 1 described in text. A_max is maximal F/F₀; A_min is minimal F/F₀. Statistically significant differences in F/F₀ values recorded before and after application of each concentration of inhibitors are indicated by asterisk: *p<0.05.