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Table 1 Primer sets used for detection of NCX in real-time RT-PCR analyses

From: Sodium-calcium exchangers in rat trigeminal ganglion neurons

Sequence

Product size

For real-time RT-PCR

 

NCX1

109 bp

F: 5′-GTGTTTGTCGCTCTTGGAACCTC-3′

 

R: 5′-CGTTGCTTCCGGTGACATTG-3′

 

NCX2

184 bp

F: 5′-CAGGTCAAGATAGTGGACGACGAA-3′

 

R: 5′-GAACTGGCTTGCCCATCTCTG-3′

 

NCX3

92 bp

F: 5′-AGCTGTCACGGTGTGAGAATGAG-3′

 

R: 5′-TGACCACAATGATGACCAGATGAA-3′

 

GAPDH

143 bp

F: 5′-GGCACAGTCAAGGCTGAGAATG-3′

 

R: 5′-ATGGTGGTGAAGACGCCAGTA-3′

 
  1. Conditions for real-time RT-PCR: 1 cycle of 42°C for 5 min, followed by 1 cycle of 95°C for 10 s, 40 cycles of 95°C for 5 s, and 60°C for 30 s; and for dissociation curve analysis: 1 cycle of 95°C for 15 s, 1 cycle of 60°C for 30 s, and 1 cycle of 95°C for 15 s. F: forward primer, R: reverse primer.