Figure 1

Pain-related behavior in WT and DKO mice. (A, B) Paw withdrawal threshold in the von Frey hair test was measured 10 days after nerve injury by using naive mice (A) or neuropathic pain model mice (B). The thresholds of PSNL-operated contralateral (Contra) and ipsilateral (Ipsi) sides were assessed in (B). Column chart shows withdrawal threshold in WT (open column) and DKO (closed column) mice (mean ± S.E.M., n = 7–10). *P < 0.05 versus the corresponding WT values (Student’s t-test). (C) Alteration of spinal PRIP-1 and PRIP-2 expression 10 days after sham operation (open column) or PSNL (close column) in naive C57BL/6 mice assessed by immunoblotting. The levels of immunoreactivity were normalized to that of β-tubulin and represented as % induction compared with the values of WT mice (means ± S.E.M., n = 4).