Blockade of p-cPLA
signals in spinal neurons after nerve injury. (a-i) Vehicle, MK-801, CP-99994, AACOCF3, BEL (each 10 nmol, i.t.) and minocycline (30 mg/ml, i.p.) were pre-treated before nerve injury, and the spinal cord were collected at 1 h after injury, which were used for the double immunohistochemistry with p-cPLA2 (green) and NeuN (red). Control as well as Lpar1- and Lpar3-deficient mice were also used in this experiment. White arrows represent NeuN-co-localized p-cPLA2 signals. The capital letter “C” and “I” represent control and nerve injury, respectively. The “veh”, “MK”, “CP”, “AA” and “mino” represent vehicle, MK-801, CP-99994, AACOCF3 and minocycline, respectively. Scale bar, 50 μm.