Figure 2
From: Modulation of TRP channels by resveratrol and other stilbenoids
Resveratrol suppresses TRPA1 currents, but not TRPV1 currents in a concentration dependent manner in transfected HEK293 cells. A: Representative traces from whole-cell patch-clamp experiments show the IAITC (AITC, 100 μM) in the absence (left) or presence (right) of resveratrol (RES, 30 μM). B: Dose–response curve showing inhibition of IAITC by RES. Each point represents relative IAITC with different concentration of RES treatment normalized to the IAITC without RES treatment (mean ± SEM, n = 6–11). IC50 = 0.75 μM. C: Concentration curves of IAITC in the absence (open circles) and presence (filled circles) of RES. Note the maximum response was inhibited without changing the EC50 (61.2 μM for AITC, 61.7 μM for RES + AITC). D: Representative traces show the I2APB (2APB, 400 μM) in the absence (left) or presence (right) of resveratrol (RES, 30 μM). E: The bars graph shows RES significantly inhibits I2APB density (pA/pF). * p < 0.05, unpaired t – test. F: Representative traces show the ICAP (capsaicin, 20 nM) in the absence (left) or presence (right) of RES. G: Bars graph shows no effect of RES on ICAP. Numbers in parenthesis indicate cells tested. AITC or capsaicin was perfused until current reaching the peak. Holding potential (Vh) = −60 mV in all experiments.