Effect of 10 μM AMI on the voltage-activation relationship of TTX-r Na
1.9 currents. A: The protocol to elicit Nav1.9 currents starting from a holding potential of −70 mV to the prepulse potential of −100 mV, and the voltage steps ranging from −80 to −20 mV in increments of +5 mV. B and C: Representative recordings of the Nav1.9 currents elicited by a series of voltage pulses using the protocol in (A) before and after perfusion with 10 μM AMI, respectively. D: The effect of 10 μM AMI on the I/V curves of Nav1.9 currents. Each point was normalized to the respective I-35 mV currents (n = 11). E: Effect of 10 μM AMI on the voltage-activation relationships of Nav1.9 currents. Each point was normalized to its respective I-35 mV of Nav1.9 currents (n = 11).