Effect of 10 μM AMI on the steady-state inactivation relationship of TTX-r Na
1.9 currents. A: The protocol to elicit steady-state inactivation of Nav1.9 currents starting from a holding potential of −70 mV, applying conditioning pulses ranging from −110 to −35 mV in increments of +5 mV, and applying a test pulse at −35 mV. B and C: Representative recordings of the Nav1.9 currents elicited by a series of test pulses using the protocol in (A) before and after exposure to 10 μM AMI, respectively. D: The effect of 10 μM AMI on the steady-state inactivation relationship of Nav1.9 currents. Each point was normalized to its respective maximal Nav1.9 currents (n = 11).